SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
SOLVED: Melting Temperature Tm = 4(GC) + 2(AT) Part VIII - Hybridization and the Southern Blot 3.0 Pts a) Calculate the melting temperature (Tm) of the following short DNA probes using these
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Melting temperature (Tm°C) and annealing temperature (Ta°C) data... | Download Table